To perform a spay, the animal is placed in a relaxed position on its back. A small, midline, abdominal incision is made between the umbilicus (belly button) and pelvis.

One of the ovaries is isolated. Once the blood vessels are tied off to prevent bleeding, the ovary is removed from the abdomen. Similarly the second ovary is removed.

Finally, after tying off the blood vessels to the uterus, the whole anatomical structure including ovaries, uterine horns and the uterus are removed through the abdominal incision. The body wall and the skin are closed using sutures.